Expression of matrix metalloproteinase-3 after brain contusion in rats.
- Author:
Xiao-chong GUO
1
;
Ru-bo LI
;
Hong-xia LIANG
;
Fu-yuan WANG
;
Xu WU
Author Information
1. Department of Forensic Pathology, School of Forensic Medicine, China Medical University, Shenyang 110001, China.
- Publication Type:Research Support, Non-U.S. Gov't
- MeSH:
Animals;
Blotting, Western;
Brain Injuries/enzymology*;
Forensic Pathology;
Immunohistochemistry;
Male;
Matrix Metalloproteinase 3/genetics*;
Random Allocation;
Rats;
Rats, Sprague-Dawley;
Time Factors
- From:
Journal of Forensic Medicine
2009;25(1):1-5
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the expression of matrix metalloproteinase-3 after brain contusion and its applicability for estimating the age of brain contusion.
METHODS:Rats had been divided into three groups: control group, sham operation group and brain contusion group. The expression of matrix metalloproteinase-3 at different time was detected by immunohistochemistry and Western blot.
RESULTS:By the immunohistochemistry, no staining was observed in control and sham operation groups. The positive staining of MMP-3 appeared 6 hours after contusion, increased gradually in 24 hours and peaked 5 days after contusion, then started to decrease, 14 days after contusion still could be observed. By the Western blot analysis, no expression of MMP-3 was detected in control and sham groups. The positive staining of MMP-3 appeared 6 hours after contusion, increased gradually and maximized 5 days after contusion, then started to decrease, 14 days after contusion still could be found.
CONCLUSION:Time-order expression of MMP-3 could be used for estimating the age of brain contusion in forensic pathology.
