A method for DNA examination on mouth mucosa exfoliative cells from toothbrush bristles.
- Author:
Yu FENG
1
;
Mi YAN
;
Yun LIU
;
Jun CHEN
Author Information
1. Institute of Forensic Science, Chongqing Public Security Bureau, Chongqing 400042, China. fy777777@126.com
- Publication Type:Journal Article
- MeSH:
DNA/analysis*;
Epithelial Cells/metabolism*;
Forensic Genetics;
Humans;
Microsatellite Repeats/genetics*;
Mouth Mucosa/cytology*;
Polymerase Chain Reaction;
Toothbrushing
- From:
Journal of Forensic Medicine
2008;24(6):435-436
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish a method for DNA examination on mouth mucosa exfoliative cells from toothbrush bristles.
METHODS:Slough-off mouth mucosa exfoliative cells were collected by bristles extracted method and direct washing method. The DNA of exfoliative cells was extracted with Chelex-100 method and DNA IQTM kit. Then all the extracted DNA took PCR amplification and STR analysis.
RESULTS:There was a significant difference between the groups with bristles extracted method and with direct washing method at the success rates of over 9 STR loci detected (P<0.05). However, there was no statistical significance between Chelex-100 method and DNA IQTM kit (P>0.05).
CONCLUSION:It is demonstrated that mouth mucosa exfoliative cells collected by bristles extracted method is more available than that with direct washing method.
