Analysis of paraquat in blood and urine by sodium borohydride/nickel chloride chemical reduction-gas chromatography/thermionic specific detector.
- Author:
Lu-Yao HUANG
1
;
Lin-chuan LIAO
;
Li-Li CHEN
;
Gui SU
;
Wen-Jia LI
;
Rong HE
;
You-Yi YAN
;
Lin YANG
Author Information
1. West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China.
- Publication Type:Journal Article
- MeSH:
Borohydrides/chemistry*;
Chromatography, Gas/methods*;
Forensic Toxicology;
Herbicides/urine*;
Humans;
Nickel/chemistry*;
Oxidation-Reduction;
Paraquat/urine*;
Sensitivity and Specificity
- From:
Journal of Forensic Medicine
2008;24(6):429-432
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish a new method for the analysis of paraquat in blood and urine by sodium borohydride/nickel chloride chemical reduction-gas chromatography/thermionic specific detector.
METHODS:An initial procedure of precipitation was performed by adding hydrochloric solution with sodium chloride and a mixture of chloroform and ethanol. Then the analyte contained in supernatant was reduced by a reduction system of sodium borohydride and nickel chloride and extracted by acetic ether. Ethyl paraquat (EPQ) was used as internal standard. GC/TSD was used to identify and quantify the analyte.
RESULTS:The limits of detection (S/N=3) in blood and urine were 0.002 and 0.004 microg/mL, respectively. The linear ranges were 0.050-30.0 microg/mL. Correlation coefficients in blood and urine were 0.999 and 0.998, respectively. The recoveries exceeded 80% both in blood and urine.
CONCLUSION:This method is applicable for quantification of paraquat in biological fluids.
