Changes of ICAM-1 mRNA expression in rats' skin and muscle after contusion.
- Author:
Jun-Hong SUN
1
;
Qiu-Xiang DU
;
Xiao-Wei WANG
;
Ying-Yuan WANG
Author Information
1. School of Forensic Medicine, Shanxi Medical University, Taiyuan 030001, China. sunjunhong146@163.com
- Publication Type:Research Support, Non-U.S. Gov't
- MeSH:
Animals;
Contusions/metabolism*;
Female;
Forensic Medicine;
Intercellular Adhesion Molecule-1/metabolism*;
Male;
Muscle, Skeletal/metabolism*;
RNA, Messenger/metabolism*;
Random Allocation;
Rats;
Rats, Sprague-Dawley;
Skin/metabolism*;
Time Factors
- From:
Journal of Forensic Medicine
2008;24(6):407-410
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the expression of intercellular adhesion molecule-1 (ICAM-1) mRNA in contused skin and muscle of rats and the relationship between the ICAM-1 expression and the wound age.
METHODS:The samples were taken at 0.5, 1, 6, 12, 18, 24 and 30 h after contusion of rats, respectively. Total RNA was extracted both from the skin and muscle samples of each group and reverse transcription polymerase chain reaction (RT-PCR) was conducted to synthesize the 1st strand cDNA. The amount of ICAM-1 mRNA in each sample was quantified using rp132 intrinsic fluorescent assay and compared by the 2 (-Delta Delta Ct) method with that from control samples.
RESULTS:After contusion the expression of ICAM-1 mRNA in skin increased rapidly and peaked at 0.5 h, at 24 h degraded to the amount that was seven times as much as the control group, then rised again. The expression of ICAM-1 mRNA in muscle increased significantly within 0.5 h and peaked at 6 h, reached the minimum at 18 h, then increased again.
CONCLUSION:It is suggested that ICAM-1 mRNA analysis may be useful for estimation of early wound age because of its time-related expression after contusion in skin and muscle.
