DNA polymorphism detection of Cannabis using amplified fragment length polymorphism.
- Author:
Jia GUO
1
;
Li PEI
;
Jian-Xiong PENG
;
Hong ZHAI
;
Gui-Qin ZHANG
;
Qin JIE
;
Zheng TU
Author Information
1. Chinese People's Public Security University, Beijing 100038, China. guojia99@tom.com
- Publication Type:Research Support, Non-U.S. Gov't
- MeSH:
Amplified Fragment Length Polymorphism Analysis/methods*;
Cannabis/genetics*;
DNA, Plant/genetics*;
Forensic Genetics;
Genetic Variation;
Polymorphism, Genetic
- From:
Journal of Forensic Medicine
2008;24(5):330-332
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To screen the AFLP primers with good diversity to distinguish various species of Cannabis.
METHODS:The AFLP was used to analyze the genetic diversity of 12 species of Cannabis using 55 primer combinations.
RESULTS:A total of 285 AFLP bands were obtained using five primer combinations with better diversity, among which 99 bands were polymorphic and 10 bands were special, with 47-76 bands amplified in each pair of primers.
CONCLUSION:AFLP may has good resolution in the diversity study of Cannabis. It may provide an essential basis for further study of the genetic diversity of Cannabis.
