Changes of RyR2 mRNA expression in rats suffering from acute myocardial ischemia.
- Author:
Ya JIE
1
;
Shao-Hua ZHU
;
Yan-Wei JIANG
;
Lei ZHANG
;
Fan FAN
Author Information
1. Department of Forensic Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
- Publication Type:Research Support, Non-U.S. Gov't
- MeSH:
Animals;
Death, Sudden, Cardiac;
Down-Regulation;
Female;
Forensic Pathology;
Male;
Myocardial Ischemia/metabolism*;
RNA, Messenger/metabolism*;
Random Allocation;
Rats;
Rats, Sprague-Dawley;
Reverse Transcriptase Polymerase Chain Reaction/methods*;
Ryanodine Receptor Calcium Release Channel/metabolism*;
Sarcoplasmic Reticulum/metabolism*
- From:
Journal of Forensic Medicine
2008;24(5):327-338
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the changes of ryanodine receptor 2 (RyR2) mRNA expression in rats suffering from acute myocardial ischemia.
METHODS:SD rats were divided randomly into normal control group, myocardial ischemia group and sudden death group. The models of myocardial ischemia and sudden cardiac death were induced by intraperitoneal injection of hypophysine. The changes of RyR2 mRNA expression in cardiac sarcoplasmic reticulum (SR) of rats suffering from myocardial ischemia were detected by fluorescent RT-PCR technique.
RESULTS:The levels of RyR2 mRNA in the myocardial ischemia group and sudden death group were significant lower than those in the control group (P<0.05).
CONCLUSION:Myocardial ischemia may induce down-regulation of cardiac SR RyR2 mRNA expression.
