DNA polymorphism detection of Papaver somniferum L using fluorescent amplified fragment length polymorphism.
- Author:
Fan LU
1
;
Bao-wen CHENG
;
Hong LI
;
Jun-jie HONG
;
Hai-Yan SUN
;
Wen-song ZHAO
;
Hong-mei YANG
;
Jin LI
;
Yu-rong ZHAO
Author Information
1. Institute of Criminal Science and Technology, Yunnan Province Police Bureau, Kunming 650021, China.
- Publication Type:Research Support, Non-U.S. Gov't
- MeSH:
Amplified Fragment Length Polymorphism Analysis/methods*;
DNA, Plant/genetics*;
Fluorescent Dyes;
Forensic Genetics;
Papaver/genetics*;
Polymorphism, Genetic
- From:
Journal of Forensic Medicine
2008;24(4):262-267
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To detect DNA polymorphism of Papaver somniferum L using fluorescent Amplified Fragment Length Polymorphism.
METHODS:Genomic DNA was isolated using the AxyPrep DNA Kit, double-digested by two restrictional endonucleases (Eco RI and Mse I) and ligated to oligonucleotide adapters. After Pre-amplification and selective amplification, the DNA fragments were separated by capillary electrophoresis using the CEQ8000 DNA Fragment Analyzer.
RESULTS:More than 20 fragments of highly polymorphic products were obtained by 8 pairs of primer from 64 selective amplifying primer pairs.
CONCLUSION:The fluorescent AFLP technique can be used to detect the DNA polymorphism of Papaver somniferum.
