Multiple amplification of 16S rRNA gene and Cytb gene in mitochondrial DNA for species identification.
- Author:
Yi YE
1
;
Jin WU
;
Hai-bo LUO
;
Zhuo WANG
;
Ying-bi LI
Author Information
1. Department of Forensic Biology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China. yeyiconan@sina.com
- Publication Type:Journal Article
- MeSH:
Animals;
Base Sequence;
Carps;
Cattle;
Chickens;
Cytochromes b/genetics*;
DNA, Mitochondrial/genetics*;
Dogs;
Female;
Forensic Genetics;
Gene Amplification;
Humans;
Male;
Molecular Sequence Data;
RNA, Ribosomal, 16S/genetics*;
Species Specificity;
Swine
- From:
Journal of Forensic Medicine
2008;24(4):259-261
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish a fluorescent multiple amplification system of 16S rRNA and Cytb genes located in mitochondrial DNA for species identification.
METHODS:A pair of primers of 16S rRNA gene and Cytb gene of the mitochondrial DNA was designed with the software Primer 5.0 to construct a multiple amplification system. The amplified products from human and five species of animals, including cattle, pig, dog, chicken and grass carp were analyzed by 310 Genetic Analyzer.
RESULTS:The amplified products of these samples showed two peaks. The common one was 358bp and the specific one different in unique species was between 231bp and 256bp.
CONCLUSION:The multiplex amplification system can exactly distinguish the species of human from five common animals.
