The expressions of HSP 70 mRNA and c-fos mRNA in the skeletal muscle and cardiac muscle of rabbits by electrocuted.
- Author:
Ye WANG
1
;
Min LIU
;
Wei-bo CHENG
;
Gui-qiong HE
;
Fan LI
;
Zhi-gang LIAO
Author Information
1. West China school of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China. yellpath007@scu.edu.cn
- Publication Type:Journal Article
- MeSH:
Animals;
Electric Injuries/metabolism*;
Forensic Pathology;
HSP70 Heat-Shock Proteins/metabolism*;
Male;
Muscle, Skeletal/metabolism*;
Myocardium/metabolism*;
Postmortem Changes;
Proto-Oncogene Proteins c-fos/metabolism*;
RNA, Messenger/metabolism*;
Rabbits;
Random Allocation
- From:
Journal of Forensic Medicine
2008;24(4):245-251
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To study the changes of HSP 70 mRNA and c-fos mRNA expression and to find a method to differentiate antemortem from postmortem electrocution.
METHODS:Fifteen New Zealand rabbits were randomly divided into three groups, the antemortem electrocution group, the postmortem electrocution group, and the control group. Each group consists of five rabbits. The levels of HSP 70 mRNA and c-fos mRNA in skeletal muscle and cardiac muscle were examined with quantitative fluorescent RT-PCR.
RESULTS:The levels of HSP 70 mRNA and c-fos mRNA in the antemortem electrocution group increased significantly (P<0.05), compared with that of the postmortem electrocution group.
CONCLUSION:The changes of HSP 70 mRNA and c-fos mRNA expression in skeletal muscle and cardiac muscle can be used as an indicator to distinguish antemortem from postmortem electrocution.
