The time-dependent changes of phospho-JNK expression during the skin incised wound healing in mice.
- Author:
Chang-yan XIONG
1
;
Da-wei GUAN
;
Mei YANG
;
Rui ZHAO
;
Ji-long ZHENG
;
Ling WANG
;
Tian-shui YU
;
Zi-hui CHENG
;
Geng-yi HU
;
Bao-li ZHU
Author Information
1. School of Forensic Medicine, China Medical University, Shenyang 110001, China.
- Publication Type:Research Support, Non-U.S. Gov't
- MeSH:
Animals;
Biomarkers;
Female;
Forensic Medicine;
JNK Mitogen-Activated Protein Kinases/metabolism*;
Male;
Mice;
Phosphorylation;
Random Allocation;
Skin/injuries*;
Time Factors;
Wound Healing;
Wounds, Penetrating/enzymology*
- From:
Journal of Forensic Medicine
2008;24(4):241-244
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the changes of phospho-JNK (p-JNK) during the incised wound healing of the skin in mice and to explore the rule of the time-dependent change of p-JNK in wound age determination.
METHODS:The changes of p-JNK expression in incised skin wound were detected by immunohistochemistry and Western blot.
RESULTS:There was a minimal baseline staining of p-JNK in control mouse skin. Changes of p-JNK expression were mainly detectable in neutrophils in the wound specimens from 3 hours to 12 hours after injury. Afterwards, the p-JNK positive cells were mostly mononuclear cells and fibroblasts between post-injury day 1 and day 5, whereas the p-JNK positive cells were mostly fibroblasts between post-injury day 7 and day 14. Morphometrically, the ratio of the p-JNK positive cells to the total increased gradually in the wound specimens from 3 hours to day 1, and maximized at day 1 with a slight decrease from post-injury day 3 to day 5. The ratio showed a second peak in the specimens of day 7, and then decreased gradually from post-injury day 10 to day 14. The changes of p-JNK expression were observed throughout the wound healing stages by Western blot as well, with a peak expression occurring between 12 hour and day 3 after injury.
CONCLUSION:p-JNK may play a pivotal role in inducing apoptosis of neutrophils, mononuclear cells, and fibroblasts during skin wound healing and meanwhile, p-JNK may be a potentially useful marker for wound age determination.
