Analysis of appropriate amount of template DNA for sinofiler kit by real time quantitative PCR technique.
- Author:
Cheng-Tao LI
1
;
Hong GUO
;
Yuan LIN
;
Yan LIU
;
Ting-Zhi QUE
;
Li LI
Author Information
1. Shanghai Key Laboratory of Forensic Medicine, Institute of Forensic Science, Ministry of Justice, P.R. China. lichengtaohla@163.com
- Publication Type:Research Support, Non-U.S. Gov't
- MeSH:
DNA/analysis*;
Forensic Medicine/methods*;
Hair/chemistry*;
Humans;
Microsatellite Repeats;
Reagent Kits, Diagnostic;
Reverse Transcriptase Polymerase Chain Reaction/methods*;
Templates, Genetic
- From:
Journal of Forensic Medicine
2008;24(2):129-133
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To explore the appropriate amount of template DNA for Sinofiler Kit.
METHODS:The DNA samples with ideally genotyped results by Sinofiler Kit were detected by real-time quantitative PCR assay.
RESULTS:It was shown that 1.29-1.51 ng of template DNA in 12.5 microL reaction volume was optimal for STR genotyping with Sinofiler Kit.
CONCLUSION:Real time quantitative PCR is an accurate and necessary technique for detection of appropriate amount of template DNA for different kits.
