DNA typing of minimal oral epithelial cell samples.
- Author:
Dian YANG
1
;
Chao LIU
;
Qu-Yi XU
;
Hui-Ying HU
;
Hong LIU
Author Information
1. Institute of Gene Engineering, Southern Medical University, Guangzhou 510515, China.
- Publication Type:Research Support, Non-U.S. Gov't
- MeSH:
DNA/analysis*;
Epithelial Cells/metabolism*;
Forensic Medicine/methods*;
Humans;
Microsatellite Repeats;
Mouth Mucosa/cytology*;
Polymerase Chain Reaction
- From:
Journal of Forensic Medicine
2008;24(2):126-128
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To seek simple and cost-effective extraction technique to improve multiplex STR amplification from minimal oral epithelial cell samples.
METHODS:One hundred DNA samples of oral epithelial cells extracted by mini system Chelex-100 method were quantitated by ABI 7500 Real Time System and were then typed with Identifiler system in ABI 3130 Genetic Analyzer.
RESULTS:The DNA contents of different categories of samples were as followings: suck pipes (0.72-116.78 ng), cup edges (2.15-142.5 ng), mouths of drink bottle (1-34.65 ng), chopsticks (3.35-26.6 ng), fruit cores (0.294-21.4 ng), and poultry bones (0.88-5.88 ng). The mean successful typing rate for gender and more than 9 STR loci was about 59.38%. Except the addition or no addition of proteinase K to the samples, all other factors-C users' variation, sample extraction methods, and qualities and properties of the samples had considerable effects on the contents of extracted DNA.
CONCLUSION:Successful STR typing can be achieved in about 60% minimal oral epithelial cell DNA samples extracted by mini Chelex system.
