Morphological analysis of developmental changes in soma area of digastric motoneurons in the rat trigeminal motor nuclei.
- Author:
Jae Hyun KIM
1
;
Mi Hwa PARK
;
Sang Kyoo PAIK
;
Su Kyung MA
;
Sang Heum BAEK
;
Duwon CHA
Author Information
1. Department of Oral & Maxillofacial Surgery, Daegu Fatima Hospital, Korea. oms@fatima.or.kr
- Publication Type:Original Article
- Keywords:
Digastric motoneuron;
Trigeminal motor nuclei;
Development
- MeSH:
Animals;
Carisoprodol*;
Horseradish Peroxidase;
Rats*;
Rats, Sprague-Dawley
- From:Journal of the Korean Association of Oral and Maxillofacial Surgeons
2005;31(2):137-142
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
To analyze the developmental changes in soma diameters of digastric motoneurons, wheat-germ agglutinin conjugated horseradish peroxidase (WGA-HRP) was injected into the digastric muscle and visualized the retrogradely HRP-labeled motoneurons through tungstate/tetramethylbenzidine (TMB) and following diaminobenzidine (DAB) reactions. The results obtained from Sprague-Dawley rats at postnatal days 1 (P1), 10 (P10) and 30 (P30) indicated as follows: firstly, soma diameters of digastric motoneurons showed unimodal distribution in all postnatal days examined; secondly, the period of P1 to P10 (period 1) showed about 2 times faster growth rate than that of P10 to P30 (period 2); thirdly, the smallest soma examined in each postnatal day exhibited slower growth rate with that of the largest one (increase ratio in soma diameters from P1 to P30, smallest vs. largest =1.62 : 1.93); Finally, relative growth rates a day showed again that period 1 had faster growth rate than that of period 2. Consequently, developmental changes in soma diameters of digastric motoneurons resulted in very different growth rates between both periods. This implies that the growth of the soma is almost completing within P10 and thereafter growing slowly. The period 1 and 2 are corresponding to sucking and sucking/masticatory period, respectively. Therefore present study providing morphological changes in soma diameters of digastric motoneurons suggests that both periods and their different growth rates of the motoneurons in each period may closely be related with each other.
