Expression of cTnT in rabbit hearts during myocardial ischemia and its postmortem stability.
- Author:
Xiao-Ming XIONG
1
;
Shi-Xiong DENG
Author Information
1. Department of Pathology, Luzhou Medical College, Luzhou 646000, China. xxm_1976@163.com
- Publication Type:Journal Article
- MeSH:
Animals;
Female;
Immunohistochemistry;
Male;
Myocardial Ischemia/metabolism*;
Myocardium/metabolism*;
Postmortem Changes;
Rabbits;
Random Allocation;
Time Factors;
Troponin T/metabolism*
- From:
Journal of Forensic Medicine
2008;24(2):90-93
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To study the postmortem stability of cTnT as well as its expression alteration, and to evaluate it in the diagnosis of early myocardial ischemia in forensic practice.
METHODS:Animal model of early myocardial ischemia was established by rabbit coronary artery ligation. The expression of cTnT in myocardium at different postmortem intervals was detected using immunohistochemistry and analyzed using imaging technique and statistics. The results were then compared between the experimental and control groups.
RESULTS:In ischemic myocardium, the expression of cTnT showed prominent focal or flaky depletion in myocardial cytoplasm with no expression detected in interstitium. The expression level showed a linear decrease with prolonged postmortem interval, and disappeared completely on day 14 after death while stored at 4 degrees C. However, there were significant differences in the expression levels of cTnT between experimental and control groups from day 1 to day 7 after death.
CONCLUSION:Immunohistochemical detection of cTnT for diagnosis of early myocardial ischemia in corpses stored at 4 degrees C must be performed within 7 days after death.
