Estimation of postmortem interval with single-voxel proton 1H-MR spectroscopy at different temperature.
- Author:
Tian-Tong YANG
1
;
Zhen-Wei LI
;
Liang LIU
;
Na ZHEN
Author Information
1. Department of Forensic Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China. yttwowo@163.com
- Publication Type:Research Support, Non-U.S. Gov't
- MeSH:
Animals;
Aspartic Acid/analysis*;
Brain Chemistry;
Choline/analysis*;
Creatine/analysis*;
Forensic Pathology;
Magnetic Resonance Spectroscopy;
Postmortem Changes;
Rabbits;
Temperature;
Time Factors
- From:
Journal of Forensic Medicine
2008;24(2):85-89
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To find an objective method for estimation of postmortem interval (PMI) with single-voxel proton MR spectroscopy at different temperature.
METHODS:Changes of metabolite peak area and peak area ratio of the N-acetylaspartate (Naa), choline (Cho) and total creatine (Cr) in the brain of 24 rabbit models were measured by 1H-MR spectroscopy (1H-MRS) at 0.5, 1, 2, 4, 6, 8, 12, 16 and 24 h, respectively after death.
RESULTS:Naa and Cr decreased continuously with the extension of PMI, and the ratios of Naa/Cr and Cho/Cr also decreased continuously with the extension of PMI within 24 h. The quadratic polynomial regression equation is y=0.0019x2-0.803x+1.4498 (R2=0.962) (Equation 1) with the Naa/Cr used as an independent variable, while the quadratic polynomial regression equation is y=-0.0024x2+0.926x+1.1777 (R2=0.986) (Equation 2) with the Cho/Cr used as an independent variable. The average deviations were about 2.10-37.90 min and 1.69-40.87 min, respectively if the PMI was estimated by Equation 1 and Equation 2, respectively.
CONCLUSION:Our study indicates that there is a strong correlation between Naa/Cr, Cho/Cr and PMI, which may be used for estimation of PMI at different temperature.
