Expressions and time-dependent changes of FAK and phospho-FAK during skin incised wound healing in mouse.
- Author:
Da-Li YANG
1
;
Da-Wei GUAN
;
Jian-Jun ZHANG
Author Information
1. School of Forensic Medicine, China Medical University, Shenyang 110001, China.
- Publication Type:Research Support, Non-U.S. Gov't
- MeSH:
Animals;
Focal Adhesion Kinase 1/metabolism*;
Mice;
Phosphorylation;
Skin/injuries*;
Time Factors;
Wound Healing
- From:
Journal of Forensic Medicine
2008;24(2):81-84
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To study the expressions of focal adhesion kinase (FAK) and phospho-FAK( p-FAK) during skin incised wound healing and the applicability of time-dependent expressions of FAK and p-FAK.
METHODS:The expression of FAK and p-FAK in cutaneous incised wound in mouse were investigated by immunohistochmeistry and Western blotting.
RESULTS:FAK and p-FAK expression were detected in polymorphonuclear cells (PMNs) in the wound and adjacent regions 3 hours post-injury. The expressions of FAK and p-FAK were detected in a large number of infiltrating PMNs and some of mononuclear cells (MNCs) from 6 to 24 hours after injury. The MNCs and fibroblastic cells (FBCs) accounted for most part of the FAK and p-FAK positive cells from 3 to 14 days after injury. The numbers of FAK-positive cells increased continuously, reaching a peak at post-injury day 3, and then started to decrease from post-injury day 5 to 14. The changes of p-FAK-positive cells were similar to that of the FAKs, and reached a peak at 12 hours after injury.
CONCLUSION:Both FAK and p-FAK displayed a time-dependent expression during skin incised wound healing in mouse, with p-FAK being superior to FAK. Both FAK and p-FAK may potentially be used as new markers for determination of the wound interval.
