Determination of ketamine and norketamine in blood and urine by high performance liquid chromatography.
- Author:
Li-Li CHEN
1
;
O Lin-Chuan LIA
;
Wen-Jia LI
;
Lu-Yao HUANG
;
You-Yi YAN
;
Lin YANG
;
Xiao-Na MA
;
Rong HE
Author Information
1. West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China.
- Publication Type:Research Support, Non-U.S. Gov't
- MeSH:
Animals;
Chromatography, High Pressure Liquid;
Female;
Ketamine/urine*;
Male;
Rats;
Rats, Wistar
- From:
Journal of Forensic Medicine
2008;24(1):38-42
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To develop a high-performance liquid chromatographic method for determination of ketamine and norketamine in blood and urine.
METHODS:The compounds were extracted from blood or urine by liquid-liquid extraction using toluene after blood or urine was adjusted pH to 14. The extracts were analyzed by HPLC.
RESULTS:Linear limits of ketamine and norketamine determination in blood ranged from 0.05 microg/mL to 10 microg/mL (R2 > 0.9993) and in urine ranged from 0.01 microg/mL to 200 microg/mL (R2 > 0.9995). Limits of detection (LODs) for ketamine and norketamine were 0.006 microg/mL and 0.003 microg/mL (S/N > or = 3), respectively. The mean extraction recovery was over 82.4% and its coefficients of variation were less than 10.0% for ketamine and norketamine. Concentration-time curves and urinary drug velocity curves of ketamine norketamine were obtained by determinations of them in blood and urine in rat using the developed method.
CONCLUSION:The method is sensitive, simple, rapid and suitable for determination of ketamine and norketamine in blood and urine for toxicological and clinical pharmaceutical analysis.
