Time-dependent expression of caspase-3 during contused skeletal muscle wound healing in rats.
- Author:
Qi WANG
1
;
Da-Wei GUAN
;
Rui ZHAO
;
Xue PENG
;
Jian-Jun ZHANG
;
Da-Li YANG
Author Information
1. School of Forensic Medicine, China Medical University, Shenyang 110001, China.
- Publication Type:Research Support, Non-U.S. Gov't
- MeSH:
Animals;
Caspase 3/metabolism*;
Contusions/enzymology*;
Forensic Pathology;
Male;
Muscle, Skeletal/injuries*;
Random Allocation;
Rats;
Rats, Sprague-Dawley;
Time Factors;
Wound Healing
- From:
Journal of Forensic Medicine
2008;24(1):5-11
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the expression of caspase-3 during contused skeletal muscle wound healing in rats and to explore the applicability of caspase-3 in wound age estimation.
METHODS:Male Sprague-Dawley rats undergone anesthesia with 2% pentobarbital sodium (45 mg/kg) were struck by a single strike at the skeletal muscle of the right leg, then were sacrificed by cervical dislocation at different post-traumatic intervals. The changes of caspase-3 activity were assessed by imunohistochemistry, Western blot and caspase-3 colorimetric assay. The skeletal muscle of non-contusion rat's were used as controls.
RESULTS:No caspase-3 was detected in skeletal muscle in the control group. The expression level of caspase-3 in the peripheral area of contused rat skeletal muscle increased gradually and maximized on the fifth day after injury, and then decreased gradually. Caspase-3 was also detected in the regenerated skeletal muscle within 7-14 days after injury.
CONCLUSION:The expression of capase-3 in the peripheral area of contused rat skeletal muscle indicated that it may play a pivotal role in apoptosis of the wounded skeletal muscle and may be used as a marker for wound age estimation. The expression of capase-3 in regenerating skeletal muscle suggests that caspase-3 activity may be used for skeletal muscle differentiation.
