The effects of heroin on intracellular free Ca2+ of rat myocardium.
- Author:
Xiao-Shan LIU
1
;
Jing-Dong WU
;
Zi-Rui HAO
;
Shui-Ping LIU
;
Zhao-Hui LI
;
Hai-Xia LI
;
Yu-Chuan CHEN
Author Information
1. Department of Forensic Medicine, Medical College of Sun Yat-sen University, Guangzhou, China. lxsa200@yahoo.com.cn
- Publication Type:Journal Article
- MeSH:
Animals;
Calcium/metabolism*;
Calcium Signaling;
Cells, Cultured;
Dose-Response Relationship, Drug;
Heroin/pharmacology*;
Microscopy, Confocal/methods*;
Microscopy, Fluorescence;
Myocytes, Cardiac/metabolism*;
Rats;
Rats, Sprague-Dawley
- From:
Journal of Forensic Medicine
2007;23(6):424-427
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To observe the effects of heroin on intracellular free Ca2+ in rat myocardium.
METHODS:The effects of heroin on intracellular free Ca2+ were observed in cultured neonatal rat myocardium by measuring intracellular free Ca2+ concentration using calcium fluorescent probe Flou-3/AM and laser scanning confocal microscope.
RESULTS:Different doses and concentrations of heroin appeared to have different effects on intracellular free Ca2+ concentrations, with a dosage dependent short linear increase in the fluorescence intensity (i.e., Ca2+ concentration) leading to [Ca2+]i peak.
CONCLUSION:Heroin could affect concentrations of [Ca2+]i in myocardium and its dosage related effect needs further investigation.
