Application of immunofluorescence and sandwich ELISA with double-antibodies in detection of human rabies.
- Author:
Lai-Jing SUN
1
;
Ge-Lin XU
;
Hua-Lin WANG
;
Jie WU
;
Zhi-Hong HU
;
Yi-Wu ZHOU
Author Information
1. Department of Forensic Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China. sunlaijing@163.com
- Publication Type:Research Support, Non-U.S. Gov't
- MeSH:
Animals;
Antibodies, Viral/analysis*;
Brain/virology*;
Enzyme-Linked Immunosorbent Assay/methods*;
Fluorescent Antibody Technique/methods*;
Hippocampus/virology*;
Humans;
Rabies/virology*;
Rabies virus/immunology*;
Rats;
Reagent Kits, Diagnostic;
Sensitivity and Specificity;
Tissue Preservation/methods*
- From:
Journal of Forensic Medicine
2007;23(6):411-413
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To explore the application of immunofluorescence and sandwich ELISA with double-antibodies in detection of human rabies.
METHODS:The cerebrum, cerebellum, brainstem, and hippocampus of four patients died of rabies identified by clinical diagnosis were collected and kept in freezer at -70 degrees C or in formaldehyde solution separately. The rat brain tissue infected by CVS strain of rabies virus was used as a positive control and the brain tissue of a patient died of acute pancreatitis was used as a negative control.
RESULTS:Rabies virus was detected in the tissues kept in freezer at -70 degrees C and the positive control but was not detected in the tissues kept in formaldehyde solution and the negative control.
CONCLUSION:Immunofluorescence and Sandwich ELISA with double-antibodies could be used in detection of human rabies. The samples should be kept in deep frozen temperature condition instead of in formaldehyde solution.
