Effects of serum from crush injury rats on vascular endothelial cell apoptosis and their potential mechanism.
- Author:
Shui-Ping LIU
1
;
Bin LUO
;
Zhao-Hui LI
;
Xiao-Shan LIU
Author Information
1. Department of Forensic Pathology, Zhongshan Medical School of Sun Yat-sen University, Guangzhou, China. liushp@mail.sysu.edu.cn
- Publication Type:Research Support, Non-U.S. Gov't
- MeSH:
Animals;
Apoptosis/drug effects*;
Atrial Natriuretic Factor/blood*;
Calcium/metabolism*;
Cattle;
Cells, Cultured;
Culture Media/chemistry*;
Disease Models, Animal;
Endothelial Cells/metabolism*;
Endothelin-1/blood*;
Extremities/injuries*;
Flow Cytometry;
Rats;
Rats, Sprague-Dawley
- From:
Journal of Forensic Medicine
2007;23(6):409-413
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the effects of serum from crush injury rats on vascular endothelial cell apoptosis and their potential mechanism.
METHODS:Bovine aorta endothelial cells were cultured in vitro and the effects of serum from crush injury rats on cell apoptosis and intracellular calcium concentration ([Ca2+]i) were observed. Meanwhile, the levels of rat blood plasma endothelin-1 (ET-1) and atrial natriuretic peptide(ANP) were measured.
RESULTS:Compared with normal rat serum treatment, the cell apoptosis rate decreased from (8.26+/-1.75)% to (2.75+/-0.90)%, while the concentration of [Ca2+]i increased from (96.98+/-3.95) to (118.79+/-3.22) nmol/L in serum from crush injury rats, respectively. The concentration of ET-1 and ANP increased significantly in crush injury rat serum.
CONCLUSION:Serum from crush injury rats could inhibit apoptosis of the vascular endothelial cells. These effects may be related to increased level of [Ca2+]i mediated by ET-1 and ANP.
