Application of diatom detection using knead pulp method.
- Author:
Guang-hua YE
1
;
Lin-sheng YU
;
Yi-gu ZHANG
Author Information
1. Department of Forensic Medicine, Wenzhou Medical College, Wenzhou 325035, China. yeghua345@126.com
- Publication Type:Journal Article
- MeSH:
Calcium/metabolism*;
Diatoms/ultrastructure*;
Drowning/diagnosis*;
Forensic Pathology;
Humans;
Kidney;
Liver;
Lung;
Microscopy/methods*;
Specimen Handling/methods*;
Tooth
- From:
Journal of Forensic Medicine
2007;23(5):355-357
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:A novel technology for detection of diatom was discussed.
METHODS:Five grams of testing sample were taken and the organics were removed using simple mechanical knead pulp method. The homogenized samples were concentrated by centrifugation, smeared, and then examined under light microscope.
RESULTS:Except for a few feather's grains, the vast majority of diatom could be identified easily with clear diatom striations. The organic diatom could also be easily detected by this methodology.
CONCLUSION:The detection of diatom using knead pulp method is not only simple and inexpensive with a higher successful rate, but also causes nearly no harm to human and environment.