Simultaneous screening for 22 poisonous alkaloids in blood by liquid chromatography-tandem mass spectrometry with multiple-reaction monitoring.
- Author:
Wei LIU
1
;
Min SHEN
;
Bao-hua SHEN
;
Ping XIANG
;
He-jian WU
Author Information
1. Department of Forensic Medicine, Shanghai Medical College, Fudan University, Shanghai 200032, China.
- Publication Type:Journal Article
- MeSH:
Aconitine/blood*;
Alkaloids/chemistry*;
Chromatography, Liquid/methods*;
Colchicine/blood*;
Forensic Medicine;
Humans;
Sensitivity and Specificity;
Strychnine/blood*;
Tandem Mass Spectrometry/methods*
- From:
Journal of Forensic Medicine
2007;23(5):349-352
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish a liquid chromatography-tandem mass chromatography (LC-MS/MS) method for the simultaneous screening for 22 poisonous alkaloids in blood.
METHODS:This method involves a liquid-liquid extraction (LLE) followed by liquid chromatography-tandem mass spectrometry with multi-ple-reaction monitoring (MRM). After blood was extracted with buprenorphine as the internal standard, the target compounds were analyzed with LC-MS/MS-ESI in the positive ionization mode.
RESULTS:Identification was based on the compound's retention time and two precursor-to-product ion transitions. The limits of detection ranged from 0.1 ng/mL to 20 ng/mL in blood.
CONCLUSION:The method was sufficiently selective and sensitive to detect poisonous alkaloids and can be applied in forensic and clinical toxicology.
