Polymorphism of five short tandem repeat systems in Chinese Han population in Chengdu.
- Author:
Ying LI
1
;
Hai-bo LUO
;
Yan-hua SONG
;
Xiao CHEN
;
Bin LONG
;
Guo-di CHEN
Author Information
1. West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China. habaer_cn@sina.com
- Publication Type:Journal Article
- MeSH:
Alleles;
Asian People/genetics*;
China/ethnology*;
Electrophoresis, Polyacrylamide Gel;
Forensic Medicine;
Gene Frequency;
Genetic Markers/genetics*;
Genotype;
Humans;
Polymerase Chain Reaction;
Polymorphism, Genetic;
Rape;
Tandem Repeat Sequences/genetics*
- From:
Journal of Forensic Medicine
2007;23(5):338-341
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To obtain population genetic data of loci D11S4951, D11S4957, GATA193H05, D2S2951, and D6S2421 in Han population in Chengdu area and to validate the value of their forensic application.
METHODS:Blood samples were collected in EDTA tubes from unrelated individuals. DNAs were extracted with Chelex-100 and were analyzed by PCR and horizontal PAGE followed by silver staining.
RESULTS:Alleles 7, 10, 8, 6 and 8 were found in 5 STR loci, respectively. No deviations from Hardy-Weinberg balance were observed. The heterozygosities observed were 0.743, 0.772, 0.833, 0.650 and 0.800, respectively. The chances of exclusion were 0.497, 0.549, 0.662, 0.356 and 0.599, and the discrimination powers were 0.863, 0.912, 0.947, 0.829 and 0.931.
CONCLUSION:All of the five loci studied may be useful markers for individual identification and paternity testing.
