A study on the time-dependent expression of caspase-6 during repair of skin contusion in rats.
- Author:
Xue PENG
1
;
Da-wei GUAN
;
Qi WANG
Author Information
1. School of Forensic Medicine, China Medical University, Shenyang 110001, China.
- Publication Type:Research Support, Non-U.S. Gov't
- MeSH:
Animals;
Apoptosis;
Blotting, Western;
Caspase 6/metabolism*;
Contusions/pathology*;
Fibroblasts/metabolism*;
Immunohistochemistry;
Male;
Monocytes/metabolism*;
Neutrophils/metabolism*;
Rats;
Rats, Sprague-Dawley;
Skin/pathology*;
Time Factors;
Wound Healing
- From:
Journal of Forensic Medicine
2007;23(5):325-331
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the expression of caspase-6 in rat skin contusion and its surrounding areas during repairment.
METHODS:Immunohistochemical SP method and Western blot technique were used to study the expression and activation of caspase-6 in rat skin contusion and its surrounding areas.
RESULTS:Weak expression of caspase-6 was detected in cytoplasms of polymorphonuchear cells (PMNs) infiltrated in the injured area at 3 hours post-contusion. The ratio of the caspase-6 positive cells was low (25.78 +/- 1.38)%. The expression of caspase-6 was increased prominently (47.70 +/- 5.14)% at 12 hours post-contusion. Almost all of the PMNs, mononuclear cells (MNCs) and fibroblastic cells (FBCs) were caspase-6 positive with both cytoplasm and nucleus staining (54.58 +/- 5.64)% on post-contusion day 3. The expression of caspase-6 decreased gradually thereafter. The expression of the 34-kDa pro-caspase-6 was detected by Western blot in both control and the post-contusion groups with time dependent dynamics.
CONCLUSION:These results suggest that caspase-6 may play a major role in trauma-induced inflammatory response. Since caspase-6 shows a timely dependent expression in PMNs, MNCs and FBCs during skin injury repair in rat, it may be used as a marker for the contusion age determination,
