A study on the time-dependent expression of phospho-p38MAPK during the skin incised wound healing in mice.
- Author:
Jian-jun ZHANG
1
;
Da-wei GUAN
;
Da-li YANG
Author Information
1. School of Forensic Medicine, China Medical University, Shenyang 110001, China. blackpet@126.com
- Publication Type:Research Support, Non-U.S. Gov't
- MeSH:
Animals;
Apoptosis;
Blotting, Western;
Disease Models, Animal;
Female;
Fibroblasts/metabolism*;
Immunohistochemistry;
Male;
Mice;
Monocytes/metabolism*;
Neutrophils/metabolism*;
Phosphorylation;
Random Allocation;
Skin/metabolism*;
Time Factors;
Wound Healing;
Wounds and Injuries/physiopathology*;
p38 Mitogen-Activated Protein Kinases/metabolism*
- From:
Journal of Forensic Medicine
2007;23(5):321-324
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the expressions of phospho-p38MAPK(p-p38MAPK) during the incised wound healing of the skin in mice and to explore the applicability of the time-dependent expressions of p-p38MAPK in the determination of wound age.
METHODS:The expression of p-p38MAPK in incised skin wound was detected by immunohistochemistry and Western blot.
RESULTS:There was a minimal baseline expression of p-p38MAPK in control mouse skin. Expression of p-p38MAPK was mainly detectable in neutrophils in the wound specimens from 3hours to 12 hours after injury. Afterwards, the p-p38MAPK positive cells were mostly mononuclear cells and fibroblasts between post-injury day 1 and day 5, whereas the p-p38MAPK positive cells were mostly fibroblasts between post-injury day 7 and day 14. Morphometrically, the ratio of the p-p38MAPK positive cells to total number of the cells increased gradually in the wound specimens aged from 3 hours to 12 hours, and maximized in the wound specimens aged 12 hours with a slight decrease at 24 hours after injury. The ratio maintained at high level from post-injury day 3 to day 5, and then decreased gradually from post-injury day 7 to day 14. The expression of p-p38MAPK was observed throughout the wound healing stages by Western blot as well with a peak expression occurring between 12hour and day 3 after injury.
CONCLUSION:Our data suggest that p-p38MAPK may play an important role in inducing apoptosis of neutrophils, mononuclear cells, and fibroblasts during skin wound healing and meanwhile, p-p38MAPK may be a potentially useful marker for wound age determination.
