Application of MiniFiler kit in forensic DNA testing of low copy number template.
- Author:
Jian-ping TANG
1
;
Dan WU
;
Chen ZHANG
;
Huai-gu ZHOU
Author Information
1. Institute of Forensic Science, Shanghai Municipal Public Security Bureau, Shanghai 200083, China.
- Publication Type:Research Support, Non-U.S. Gov't
- MeSH:
Alleles;
DNA Fingerprinting/methods*;
Forensic Genetics/methods*;
Genotype;
Humans;
Polymerase Chain Reaction/methods*;
Reagent Kits, Diagnostic;
Sensitivity and Specificity;
Tandem Repeat Sequences;
Templates, Genetic
- From:
Journal of Forensic Medicine
2007;23(4):304-306
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To detect low copy number of DNA samples by using a newly launched commercial miniSTR detection kit (MiniFiler) in forensic practice.
METHODS:Low concentration and/or challenged forensic DNA samples were analyzed according to protocols provided by the manufacturer (Applied Biosystems, Foster City, USA).
RESULTS:DNA samples as low as 10 pg could be amplified by MiniFiler kit, and the optimal DNA quantity was 40 pg or above.
CONCLUSION:MiniFiler kit can be used for analysis of low copy number STR.
