Isolating sperm cells and preparing DNA from mixture sample.
- Author:
Xin LI
1
;
Lan HU
;
Xue-fei FENG
;
Xiao LIU
Author Information
1. Chinese Peoples Public Security University, Beijing 100038, China. LXcomcn88@163.com
- Publication Type:Journal Article
- MeSH:
DNA/isolation & purification*;
Forensic Medicine/methods*;
Humans;
Male;
Nucleic Acid Amplification Techniques;
Spermatozoa/chemistry*;
Tandem Repeat Sequences
- From:
Journal of Forensic Medicine
2007;23(4):286-289
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish a method of preparing and analyzing DNA of sperm cells from sample mixtures.
METHODS:Sperm cells were captured by micromanipulation method, DNA template was prepared from these sperm cells, and then amplified by multiple displacement amplification (MDA).
RESULTS:High-yield of highly integrated DNA was produced by MDA from very small amount of DNA template of 20 sperm cells. The DNA could be amplified at least 30,000-fold, and the length of most the DNA segments were greater than 15 kb, providing sufficient amount of short tandem repeat (STR) products for DNA genotyping.
CONCLUSION:The micromanipulation method is efficient to isolate sperm cells from sample mixtures and multiple displacement amplification can provide sufficient amount of DNA products for STR genotyping. The method seems to be feasible in detecting DNA from rape cases.
