Molecular genetic analysis of Diego blood group Dia and Dib in Chinese Han population.
- Author:
Bao-cheng YANG
1
;
Yu-qing SU
;
Qiong YU
;
Tian-li WEI
;
Da-cheng LI
;
Yan-lian LIANG
Author Information
1. Shenzhen Blood Center, Shenzhen Institute of Transfusion Medicine, Shenzhen 518035, China. yangbaocheng2004@163.com
- Publication Type:Research Support, Non-U.S. Gov't
- MeSH:
Asian People/genetics*;
Base Sequence;
Blood Donors;
Blood Group Antigens/metabolism*;
Blood Grouping and Crossmatching/methods*;
China/ethnology*;
Exons/genetics*;
Genotype;
Humans;
Molecular Sequence Data;
Phenotype;
Polymerase Chain Reaction/methods*;
Sequence Analysis, DNA
- From:
Journal of Forensic Medicine
2007;23(4):283-285
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To study the molecular genetic background of Diego blood group in Chinese Han population.
METHODS:A total of 2990 blood samples from unrelated blood donors were phenotyped for Dia and Dib by serological method. Twenty randomly selected samples of Di(a-b+) type and all of the samples of rare Di(a+b-) phenotype by screening were genotyped by PCR-SSP and direct DNA Sequencing.
RESULTS:Of the 2990 samples identified by serological method, 2821 were Di(a-b+), 167 were Di(a+b+) and 2 were Di(a+b-). All of the 20 randomly-selected samples with Di(a-b+) phenotype were DI2DI2 homozygote by PCR-SSP genotyping, with nucleotide C at nt position 2561 in exon 19 by direct sequencing of the DI gene. The 2 samples of rare Di (a+b-) phenotype were both the DI1DI1 homozygote, with nucleotide T at nt position 2561 in exon 19.
CONCLUSION:Our results indicate that the expression of Dia and Dib antigens in Chinese Han population most likely result from a single nucleotide T to C substitution at nucleotide position 2561 in exon 19 of the DI gene, which subsequently leads to an amino acid 854 change from Pro to Leu.
