Detecting ABO blood type of bloodstain with fluorescent antibody method.
- Author:
Rui-Heng WANG
1
;
Li-Qiang YAN
;
Wei-Jian YU
;
Xiao-Wei LIU
;
Shu-xian QU
Author Information
1. Law School of Liaoning Normal University, Dalian 116029, China.
- Publication Type:Research Support, Non-U.S. Gov't
- MeSH:
ABO Blood-Group System/immunology*;
Antibodies, Monoclonal/blood*;
Antigen-Antibody Reactions;
Blood Group Antigens/blood*;
Blood Stains;
Fluorescent Antibody Technique/methods*;
Forensic Medicine/methods*;
Humans;
Sensitivity and Specificity
- From:
Journal of Forensic Medicine
2007;23(2):127-129
- CountryChina
- Language:Chinese
-
Abstract:
UNLABELLED:OBJECTIVE To explore the advantage and feasibility of fluorescent antibody method for detection of blood type in biological material.
METHODS:According to theory of specific binding of antigen and antibody, at first the anti-A monoclonal antibody (MA) and anti-B MA were labeled with the fluorescent, then fluorescent-labeled antibodies (FLA) were bound with corresponding biological material (such as bloodstain) in the optimum condition, finally the ABO blood type of bloodstain was determined under microscope fluorescent.
RESULTS:The fluorescent antibody method is highly sensitive, accurate and simple.
CONCLUSION:The fluorescent antibody method is an accurate and reliable method for detection of ABO blood type in biological material.
