Sequence-length polymorphism research of C-stretch in mitochondrial DNA hyper-variant region.
- Author:
Ya TUO
1
;
Qing-Bo LIU
;
Sheng-Bin LI
Author Information
1. The National Laboratory of the Ministry of Health for Forensic, College of Medicine, Xi'an Jiaotong University, Xi'an 710061, China. tanny@stu.xjtu.edu.cn
- Publication Type:Journal Article
- MeSH:
Alleles;
China;
DNA Primers;
DNA, Mitochondrial/genetics*;
Gene Frequency;
Genetics, Population;
Genotype;
Haplotypes/genetics*;
Humans;
Polymerase Chain Reaction;
Polymorphism, Genetic;
Sequence Analysis, DNA
- From:
Journal of Forensic Medicine
2007;23(2):123-126
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the polymorphism of C-stretch in mitochondrial DNA hyper-variant region and the values in forensic science.
METHODS:The gene frequency were detected in such two loci of nt16180 and nt310 by using PCR-sequencing technique with two pair of primers according to the reference.
RESULTS:The length of PCR products were 807 bp and 962 bp respectively. Seven genotypes in each locus were detected. AAAACCCCCTCCCC allele amounted to 87.72% of all nt16180 genotype, while AAAACCCCCCCCCCCCC was firstly reported in Han population. CCCCCCCCTCCCCCC allele amounted to 60.53% of all nt310 genotype. 15 haplotypes were detected among 114 samples. The GD value was 0.6309. The AAAACCCCCTCCCC-CCCCCCCCTCCCCCC haplotype was the most high frequency and amounted to 57.89%.
CONCLUSION:The obtained basic genetic data in two loci of nt16180 and nt310 of mtDNA will be useful for forensic science.
