Detection of gamma-hydroxybutyrate (GHB) in beverages.
- Author:
Wei LIU
1
;
Min SHEN
;
Ping XIANG
;
Jun BU
;
He-Jian WU
Author Information
1. Institute of Forensic Sciences, Ministry of Justice, P.R. China, Shanghai 200063, China.
- Publication Type:Research Support, Non-U.S. Gov't
- MeSH:
Anesthetics, Intravenous/analysis*;
Beverages/analysis*;
Forensic Medicine/methods*;
Gas Chromatography-Mass Spectrometry/methods*;
Humans;
Hydrogen-Ion Concentration;
Reproducibility of Results;
Sensitivity and Specificity;
Sodium Oxybate/chemistry*;
Substance Abuse Detection/methods*
- From:
Journal of Forensic Medicine
2007;23(2):120-129
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish an analytical method for the determination of GHB in beverages using GC/MS and LC/MS/MS.
METHODS:After beverage samples with GHB-d6 as the internal standard were extracted with ethyl acetate, then the extracts were derivatized with N,O-bis(trimethylsilyl)trifluoroacetamide (BSTFA), at last the derivateized extracts analyzed by gas chromatography- mass spectrometry. After beverage samples with GHB-d6 as the internal standard were diluted by mobile phase then directly analyzed by LC/MS/MS. Results The limit of detection was 0.2 microg/mL and both relative standard deviations for between-day and within-day assays were < 8.54% in GC/MS. The limit of detection was 2 microg/mL and both relative standard deviations for between-day and within-day assays were <8.62% in LC/MS/MS. Conclusion These methods of qualitative and quantitative analysis were found to be sensitive, accurate, rapid and suitable for the forensic toxicology to test of GHB in real cases.
