Expression of EDA-fibronectin in injured human skin fibroblast and its potential application in estimation of wound healing.
- Author:
Meng HE
1
;
Song YU
;
Ai-Min XUE
;
Yi-Wen SHEN
;
Long CHEN
;
Yun-Ju GU
;
Zi-Qin ZHAO
Author Information
1. Department of Forensic Medicine, Shanghai Medical Collage, Fudan University, Shanghai 200032, China.
- Publication Type:Research Support, Non-U.S. Gov't
- MeSH:
Blotting, Western;
Cells, Cultured;
Fibroblasts/metabolism*;
Fibronectins/metabolism*;
Forensic Pathology;
Humans;
Immunohistochemistry;
RNA, Messenger/metabolism*;
Skin/cytology*;
Time Factors;
Wounds and Injuries
- From:
Journal of Forensic Medicine
2007;23(2):94-96
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:[corrected] To study the expression of EDA-FN variant in injured human skin fibroblast and its relation to wound healing.
METHODS:Human skin fibroblast cells were separated from human skin and cultured and monoclonal antibody to fibronectin [IST-9] was applied to amplify target segments.
RESULTS:Trace amount of EDA-FN was detected in the culture cells. The expression was increased within 8 hours after the injury.
CONCLUSIONS:The expression of EDA-FN would be an ideal marker for estimation of wound healing
