The correlation between the DNA content in human thyroid follicular epithelial cells and the postmortem interval.
- Author:
Guang-Mu REN
1
;
Cai-Liang JING
;
Ji LIU
;
Ya-Fang WANG
;
Ying-Yuan WANG
Author Information
1. School of Forensic Medicine, Shanxi Medical University, Taiyuan 030001, China.
- Publication Type:Research Support, Non-U.S. Gov't
- MeSH:
Adult;
Cadaver;
Cell Nucleus/metabolism*;
DNA/metabolism*;
Epithelium/metabolism*;
Female;
Forensic Pathology;
Humans;
Image Processing, Computer-Assisted/methods*;
Male;
Middle Aged;
Postmortem Changes;
Rosaniline Dyes;
Staining and Labeling/methods*;
Thyroid Gland/metabolism*;
Time Factors;
Young Adult
- From:
Journal of Forensic Medicine
2007;23(2):92-96
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To study the relationship between the DNA content in follicular epithelial cells of the human thyroid and postmortem interval (PMI).
METHODS:Changes of the DNA content in thyroid follicular epithelial cells at different PMI were determined by Methyl Green-Pyronin (MGP) stain combined with an image analysis technique.
RESULTS:The average DNA content in the thyroid follicular epithelial continued to decrease with increased PMI. The coefficient of determination for DNA content with average gray, aimed area, aimed area ratio and positive index in follicular epithelial cells were 0.960, 0.987, 0.988 and 0.990, respectively.
CONCLUSION:There is an apparent correlation between the average DNA content of follicular epithelial cells and the PMI. MGP stain combined with an image analysis technique seems to be a useful means to study the degradation of DNA in thyroid follicular epithelial cells.
