Haplotype and heteroplasmy in the HV II region of mtDNA in human blood detected by PCR-DGGE.
- Author:
Xian-dun ZHAI
1
;
Dai-xin HUANG
;
Hui YIN
;
Qin-en YANG
Author Information
1. Department of Forensic Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China. sanmao506@163.com
- Publication Type:Journal Article
- MeSH:
Asian People/genetics*;
China/ethnology*;
DNA, Mitochondrial/genetics*;
Electrophoresis, Polyacrylamide Gel/methods*;
Genetic Heterogeneity;
Genetics, Population;
Haplotypes;
Humans;
Mutation;
Polymerase Chain Reaction/methods*;
Sequence Analysis, DNA
- From:
Journal of Forensic Medicine
2007;23(1):30-32
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish a simple and effective technique for detecting haplotype and heteroplasmy of mtDNA, and investigate their frequencies in Chinese Han population.
METHODS:The fragments from 29-290 nt of mtDNA HV II from peripheral leukocytes of 200 unrelated Wuhan Han individuals were analyzed by using PCR-DGGE technique.
RESULTS:Seventeen haplotypes were found in the range of 29-290 nt, and the haplotype diversity (HD) was 0.8844. The heterogeneity was observed from 4 individuals, and its frequency was 2%.
CONCLUSION:PCR-DGGE is a simple, sensitive and effective technique in analyzing polymorphism and heteroplasmy of mtDNA, and can be used in forensic practice.
