Expression of beclin1 and LC3 after rat's skin contusion.
- Author:
Feng-ping YAN
1
;
Yi-jiu CHEN
;
Xiao-hua HUANG
Author Information
1. Department of Forensic Medicine, Shanghai Medical College, Fudan University, Shanghai 200032, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Apoptosis Regulatory Proteins/metabolism*;
Autophagy;
Beclin-1;
Contusions/pathology*;
Forensic Medicine;
Immunohistochemistry;
Male;
Microtubule-Associated Proteins/metabolism*;
Random Allocation;
Rats;
Rats, Sprague-Dawley;
Skin/pathology*;
Staining and Labeling;
Time Factors;
Wounds and Injuries/pathology*
- From:
Journal of Forensic Medicine
2007;23(1):11-13
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:This study aimed at examining the expression of Beclin1 and LC3 in the skin of rat after contusive injury and at observing the process of autophagy in response to injury or trauma. Their significance in diagnosis of trauma and application in estimation of the post injury time interval were also investigated.
METHODS:Immunohistochemistry and imaging analysis were used to examine the expression level of Beclinl and LC3 at the injury site of the rat skin at different post-traumatic times.
RESULTS:Expression of Beclin1 and LC3 in normal rat skin or immediately after injury maintained at a very low level, but their level started to elevate 6 hours after injury and showed increasing strong expression at post injury time of day 3, day 5 and day 7.
CONCLUSION:Over-expression of Beclin1 and LC3 as well as increased autophagy, a response to injury, can be observed in the rat skin at the injury site. The expression level of Beclin1 and LC3 starts to increase 6 hours and shows a linear increase up to 7 days after injury. Beclin1 and LC3 may serve as a marker for estimation of the post injury time interval.
