Expression of synaptophysin in experimental diffuse brain injury.
- Author:
Shuan-liang FAN
1
;
Ping HUANG
;
Yan-feng LIU
;
Pin ZHANG
;
Zhen-yuan WANG
Author Information
1. Department of Forensic Science, Medical College, Xi'an Jiaotong University, Xi'an 710061, China. xafansl@mail.xjtu.edu.cn
- Publication Type:Research Support, Non-U.S. Gov't
- MeSH:
Animals;
Brain/pathology*;
Brain Injuries/pathology*;
Cerebral Cortex/pathology*;
Diffuse Axonal Injury/pathology*;
Disease Models, Animal;
Immunohistochemistry;
Intracranial Hemorrhage, Traumatic/pathology*;
Neurons/pathology*;
Rats;
Rats, Sprague-Dawley;
Staining and Labeling;
Synapses/pathology*;
Synaptophysin/metabolism*;
Time Factors
- From:
Journal of Forensic Medicine
2007;23(1):8-13
- CountryChina
- Language:Chinese
-
Abstract:
OBJECT:To investigate the changes in the expression_level of synaptophysin following diffuse brain injury (DBI) in rats and to correlate the changes of the synaptophysin expression_level with the post injury time interval.
METHODS:Wister rats were used as a DBI model induced by Marmarou method. The changes of synaptophysin immunoreactivity on coronal sections of the rats sampled at different post-injury time intervals were used as a marker. The densitometry of the synaptophysin immunoreactivity was documented by imaging technique and analyzed by SPSS software.
RESULTS:The expression level of synaptophysin in DBI rats showed dynamic changes following DBI as well as during the repairing period.
CONCLUSION:The changes of synaptophysin level may be used as a marker for estimation of the post injury time interval in DBI.
