Induction of hypoxia-inducible factor-1alpha in two kinds of rats asphyxiation death models.
- Author:
Bei-lei ZHANG
1
;
Zhi-hui YANG
;
Peng RAN
;
Wei-bo LIANG
;
Bin ZHOU
;
Geng-qian ZHANG
;
Mei-li LU
;
Lin ZHANG
Author Information
1. Department of Forensic Biology, West China School of Preclinical and Forensic medicine, Sichuan Univesity, Chengdu 610041, China. xiangzecai@gmail.com
- Publication Type:Research Support, Non-U.S. Gov't
- MeSH:
Animals;
Asphyxia/metabolism*;
Disease Models, Animal;
Female;
Hypoxia-Inducible Factor 1, alpha Subunit/metabolism*;
Hypoxia-Ischemia, Brain/metabolism*;
Immunohistochemistry;
Kidney/pathology*;
Liver/pathology*;
Lung/pathology*;
Male;
Myocardium/pathology*;
Nitrogen/poisoning*;
Random Allocation;
Rats;
Rats, Sprague-Dawley;
Time Factors
- From:
Journal of Forensic Medicine
2007;23(1):4-7
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the expression of hypoxia-inducible factor 1-alpha (HIF1-alpha) in the heart, lung, liver and kidney in rats died of two typical models of asphyxia.
METHODS:Two asphyxia models were made and tissue samples of the dead rats were collected from different groups at various postmortem duration. The expression and the changes of HIF1-alpha in various tissues were examined by immunohistochemistry and image analysis techniques. Results Significant expression of HIF1-alpha was observed in the myocardial fibers, kidney cells, liver cells and lung cells in both asphyxia models, but not in the control group. The expression of HIF1-alpha in various tissues in the rat died of nitrogen gas breathing was found in the nuclei at 0 hour and the expression level decreased gradually thereafter. The HIF1-alpha expression level and duration in various tissues of the rat died of hanging were higher and longer than that of the former group, with a peak of the expression level observed 6 hours after death, and then started to decline in all tissues except the heart where the expression still showed an increase 24 hours after death. The control groups showed a steady expression in the cytoplasm but not in the nuclei.
CONCLUSION:HIF1-alpha appears to be a valuable biomarker in the diagnosis of asphyxia within 24 hours after death.
