Comparative study of two methods of DNA extraction from different colour costal cartilage in STR genotyping.
- Author:
Qing-wen XU
1
;
Dan WU
;
Wei HU
Author Information
1. Shanghai Institute of Criminal Science and Technology, Shanghai 200083, China.
- Publication Type:Journal Article
- MeSH:
Adolescent;
Adult;
Aged;
Autopsy;
Cartilage;
DNA/isolation & purification*;
DNA Fingerprinting/methods*;
Forensic Anthropology/methods*;
Humans;
Middle Aged;
Polymerase Chain Reaction/methods*;
Resins, Synthetic/chemistry*;
Ribs;
Tandem Repeat Sequences;
Young Adult
- From:
Journal of Forensic Medicine
2006;22(6):436-437
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To compare the effectiveness of two methods of DNA extraction from different colour costal cartilages in STR genotyping.
METHODS:DNA fragments were extracted from costal cartilages of different colour of 30 corrupt corpses using Chelex-100 and Phenol-Chloroform methods. STR loci were analyzed by ABI 3100 genetic analyzer after PCR amplification by using Profiler Plus kit.
RESULTS:STR loci were completely detected in all 30 samlpes of costal cartilages when using Phenol-Chloroform DNA extraction methods. While using Chelex-100 methods, all STR loci were identified in 22 cases (11 white, 8 light-yellow and 3 yellow cases). Partial STR loci were detected in 7 cases (3 yellow and 4 yellow-brown cases) and none in 1 brown-black cases.
CONCLUSION:Suitable DNA extracion methods will be chosen depends on the colour of costal cartilage. Phenol-Chloroform methods is more effective in STR genotyping especially in dark colour costal cartilage cases.
