Expression of HIF1-alpha on myocardium and lung in rats model of asphyxia death.
- Author:
Geng-qian ZHANG
1
;
Bin ZHOU
;
Bing DU
;
Zhi-hui YANG
;
Bei-lei ZHANG
;
Yin-hua ZHU
;
Lin ZHANG
Author Information
1. Department of Forensic Biology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China. zgengqian@163.com
- Publication Type:Research Support, Non-U.S. Gov't
- MeSH:
Animals;
Asphyxia/pathology*;
Disease Models, Animal;
Female;
Hypoxia-Inducible Factor 1, alpha Subunit/metabolism*;
Hypoxia-Ischemia, Brain/metabolism*;
Immunohistochemistry;
Lung/pathology*;
Male;
Myocardium/pathology*;
RNA, Messenger/metabolism*;
Random Allocation;
Rats;
Rats, Sprague-Dawley;
Reverse Transcriptase Polymerase Chain Reaction;
Time Factors;
Tissue Distribution
- From:
Journal of Forensic Medicine
2006;22(6):407-410
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the expression of HIF1-alpha in heart and lung tissue died from asphyxia.
METHODS:The rats model of asphyxia death was constructed by hanging, different asphyxia groups and control group sets were made according the postmortem time (0,2,6,24 h), immunohistochemistry and half-quantitative RT-PCR methods were used to investigate expression of HIF1-alpha and mRNA changes on heart and lung tissue.
RESULTS:The positive staining of HIF1-alpha could be observed in the myocardium and lung tissue. Significant differences were found between the groups of asphyxia and their corresponding control group. HIF1-alpha expression was found in all the asphyxia groups while it was only expressed in the control groups of 2 h, 6 h and 24 h. Nucleic positive staining could be detected in all the asphyxia groups but none was found in the control groups. RT-PCR showed that the expression of mRNA between 0 h asphyxia group and 0 h control group were equal in both cardic muscle and lung, but elevated expression in groups of 2,6,24h compared to their control groups.
CONCLUSION:The nuclear positive staining of HIF1-alpha in heart and lung can be a special character of suffocation death.
