Expression of cathepsin-B and -D in rat's brain after traumatic brain injury.
- Author:
Yan-bo ZHANG
1
;
Xi-ping CHEN
;
Lu-yang TAO
;
Zheng-hong QIN
;
Sheng-xing LI
;
Li YANG
;
Ju YANG
;
Yun-ge ZHANG
;
Ran LIU
Author Information
1. Department of Forensic Medicine of Suzhou University, Suzhou 215123, China.
- Publication Type:Research Support, Non-U.S. Gov't
- MeSH:
Animals;
Brain/pathology*;
Brain Injuries/pathology*;
Caspase 3/metabolism*;
Cathepsin B/metabolism*;
Cathepsin D/metabolism*;
Disease Models, Animal;
Forensic Pathology;
Hippocampus/pathology*;
Immunohistochemistry;
Lysosomes;
Male;
Neurons/metabolism*;
Rats;
Rats, Sprague-Dawley;
Time Factors
- From:
Journal of Forensic Medicine
2006;22(6):404-410
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To study the expression of cathepsin-B and -D in different time point after traumatic brain injury.
METHODS:Traumatic brain injury (TBI) model was established on rats, cathepsin-B and cathepsin-D immunofluorescence staining and confocal microscope analysis were performed. Positive cells were counted by confocal microscope and image analysis techniques were used to determine the morphological changes in each group.
RESULTS:Immunofluorescence staining results showed that cathepsin-B was activated 1 hour after TBI while cathepsin-D was not activated until 12hour after TBI. Both of them got to their peak during 4 to 8days, and kept a high level of activating 32days after TBI. Cathepsin-B and -D positive cells did not merge with caspase-3 positive cells until 6 h after TBI.
CONCLUSION:Cathepsin-B and -D could be the diagnostic markers of TBI and can estimating time course of lateral TBI. They blocked caspase-3 activation at the beginning period after TBI and started to promote cell death with caspase-3 6 h after TBI.
