Relationship between CO II gene of mtDNA of Lucilia sericata and latitude interval.
- Author:
Ji-feng CAI
1
;
Ling-mei LAN
;
Yao-qing CHEN
;
Zhen-xia WANG
;
Yun-feng CHANG
;
Wei-bo LIANG
;
Jian-guo DONG
;
Zhi-gang LIAO
Author Information
1. School of Basic Medical Sciences, Central South University, Changsha 410013, China. cjf_jifeng@163.com
- Publication Type:Journal Article
- MeSH:
Animals;
DNA, Mitochondrial/genetics*;
Electron Transport Complex IV/genetics*;
Forensic Medicine/methods*;
Genetics, Population;
Geography;
Muscidae/genetics*;
Phylogeny;
Polymerase Chain Reaction/methods*;
Species Specificity;
Weather
- From:
Journal of Forensic Medicine
2006;22(6):401-403
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To deduce the region that the geographical species of Lucilia sericata come from and determine the scene of crime (SOC) based on the gene analysis of mtDNA CO II.
METHODS:A 635 bp region for CO II of 4 Lucilia sericata (belong to 2 geographical species) were collected and sequenced, compared with the data of GenBank. A neighbour-joining tree with the Tamura and Nei model was constructed by MEGA2.1 package. The number of inherit intervals of inner-species were analyzes by Kimura's two-parameter model and used for construction the relationships between hereditary and latitude interval by SPSS10.5 soft.
RESULTS:It showed that they had the relationships between inherit and latitude interval for the 8 geographical species of Lucilia sericata for CO II.
CONCLUSION:This method can be the evidence deducing the region that the geographical species of Lucilia sericata come from and further to determine the scene of crime (SOC).