Analysis of 37 drugs in whole blood by HPLC after solid phase extraction.
- Author:
Chen LIANG
1
;
Yu-Rong ZHANG
;
Qi-Yun JIN
;
You-Mei GUO
Author Information
1. Shanghai Institute of Criminal Science and Technology, Shanghai 200083, China.
- Publication Type:Journal Article
- MeSH:
Chromatography, High Pressure Liquid/methods*;
Doxapram/isolation & purification*;
Doxepin/isolation & purification*;
Estazolam/isolation & purification*;
Forensic Medicine;
Humans;
Morphine/isolation & purification*;
Papaverine/isolation & purification*;
Pharmaceutical Preparations/isolation & purification*;
Prazosin/isolation & purification*;
Procaine/isolation & purification*;
Reproducibility of Results;
Sensitivity and Specificity;
Solid Phase Extraction/methods*;
Solvents/chemistry*
- From:
Journal of Forensic Medicine
2006;22(5):349-352
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To develop a specific, sensitive, reproducible SPE-HPLC method for the determination of 37 drugs in whole blood.
METHODS:With the doxapram as internal standard, Oasis column was used to extract drugs from whole blood. Two kinds of mobile phases were used in this study. Separations were achieved by a LiChrospher 100 RP-C18 (250 mm x 4.0 mm x 5 microm) column kept at 50 degrees C, the DAD detector was set at 230 nm and 250 nm.
RESULTS:The limit of detection were 1-30 ng/mL. The method showed excellent linearity and the linear correlation coefficient was > or =0.997 98. The relative standard deviation for between-day and within-day assay were <10%.
CONCLUSION:The method is effective, simple, reliable and has been used in real cases.
