Apoptosis in myocardial cells after macleaya cordata total alkaloids poisoning in rats.
- Author:
De-Yu ZHANG
1
;
Jian-Hua ZHU
;
Wei-Zhi ZHAO
;
Mao-Wang WU
;
Yong-Liang HU
;
Li-Bao CHENG
;
Yong-Hong LI
Author Information
1. Department of forensic medicine, Wannan medical college, Wuhu 241001, China.
- Publication Type:Research Support, Non-U.S. Gov't
- MeSH:
Acute Disease;
Animals;
Apoptosis/drug effects*;
Cell Count;
Disease Models, Animal;
Female;
Immunohistochemistry;
In Situ Nick-End Labeling;
Male;
Myocardium/pathology*;
Myocytes, Cardiac/drug effects*;
Papaveraceae/chemistry*;
Papaverine/poisoning*;
Random Allocation;
Rats;
Rats, Sprague-Dawley;
Staining and Labeling;
Time Factors
- From:
Journal of Forensic Medicine
2006;22(5):330-332
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To observe pathological changes and apoptosis in rats myocardial cells after Macleaya cordata total alkaloids poisoning, and to provide some references for Macleaya cordata total alkaloids poisoning detection.
METHODS:An experimental model of Macleaya cordata total alkaloids poisoning was established, and the technology of TUNEL staining was used.The results were analyzed by computer image analysis competitive system.
RESULTS:Quantities of apoptosis in myocardial cells in poisoning groups were much more than those in the control groups at different tages (P<0.01). In addition the quantities of apoptosis were different after different poisoning duration.
CONCLUSION:Although clinical symptoms was not obvious and could not be detected by poison analysis. Pathological changes induced by Macleaya cordata total alkaloids could be found through the apoptosis detection.
