RHCE genotyping in Chinese Han from north and Li from south.
- Author:
Zhi-Gang SUN
1
;
Mei DING
;
Bao-Jie WANG
;
Hong-Wu HUANG
Author Information
1. School of Forensic Science, China Medical University, Shenyang 110001, China.
- Publication Type:Journal Article
- MeSH:
Alleles;
Asian People/genetics*;
China/ethnology*;
DNA/blood*;
DNA Primers;
Gene Frequency;
Genetics, Population;
Genotype;
Humans;
Polymerase Chain Reaction/methods*;
Rh-Hr Blood-Group System/genetics*
- From:
Journal of Forensic Medicine
2006;22(4):271-274
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish a method which processes RHCE genotyping with PCR.
METHODS:Using PCR-SSP to detect RHCE genotype in 200 cases of Han population in north of China and Li population in south of China and detecting 5 samples of parentage testing at the same time.
RESULTS:The results of RHCE genotyping in individuals of two populations are completely accorded with the results of serology typing. And the distribution of RH genotypic frequency in Han population in north China is: RHCCEE 1, RHCCEe 3, RHCCee 88, RHCcEE 4, RHCcEe 20, RHCcee 54, RHccEE 1, RHccEe 22, RHccee; The distribution of RH genotypic frequency in Li populatin in south China is RHCCEE 2, RHCCEe 2, RHCCee 106, RHCcEE 7, RHCcEe 62, RHCcee 10, RHccEE 3, RHccEe 8. The results of RHCE genotype detecting of parentage testing samples are accorded with the results of associated identification of 13 STR loci.
CONCLUSION:PCR-SSP technology can exactly detect RHCE genotype in individuals of Han population in north China and Li population in south China.
