Changes of adenylate cyclase on cerebral regions related to mophine dependence in rats.
- Author:
Shi-Jun HONG
1
;
Jun-Lin LI
;
Li-Hua LI
;
Yong-Qiang QU
;
Yong-He ZHAO
Author Information
1. The Faculty of Forensic Medicine, Kuming Medical College, Kuming 650031, China. hongshijunwood@sohu.com
- Publication Type:Research Support, Non-U.S. Gov't
- MeSH:
Adenylyl Cyclases/metabolism*;
Animals;
Brain/pathology*;
Cerebral Cortex/enzymology*;
Disease Models, Animal;
Female;
Hippocampus/enzymology*;
Male;
Morphine Dependence/pathology*;
Periaqueductal Gray/enzymology*;
Rats;
Rats, Sprague-Dawley;
Substance Withdrawal Syndrome/metabolism*;
Time Factors
- From:
Journal of Forensic Medicine
2006;22(4):254-257
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To observe the changes of adenylate cyclase(AC) on cerebral regions related to morphine dependence in rats and investigate the relationship between the enzymological changes and the mechanism of morphine dependence.
METHODS:The technique of enzyme-histochemistry was used to detect the variations of AC of special seven cerebral regions including frontalis cortex, lenticula, corpus amygdaloideun, substantia nigra, hippocampus, periaqueductal gray and locus coerleus in morphine dependent rats. The enzymological changes were observed by optical microscope. Changes of gray degree of these cerebral regions were also observed by using the image analysis system.
RESULTS:Compared with those in control group, the contents of AC in morphine dependent groups were increased.
CONCLUSION:The contents of AC are increase in those regions. The mechanism of morphine dependence close related to the increasing of AC. The correlation of the mechanism of morphine dependence and up-regulation of AC/cAMP-PKA system is discussed.
