Expressions of Bcl-2 and Caspase-3 in the internal organs of rats when tetramine was administered.
- Author:
Ye WANG
1
;
Li-Hua SHEN
;
Wei-Jie CHEN
;
Min LIU
;
Fan LI
;
Zhi-Gang LIAO
Author Information
1. West China Preclinical and Forensic Medicine School, Sichuan University, Chengdu 610041, China. wangyh11@163.com
- Publication Type:Research Support, Non-U.S. Gov't
- MeSH:
Animals;
Bridged-Ring Compounds/poisoning*;
Caspase 3/metabolism*;
Dose-Response Relationship, Drug;
Immunohistochemistry;
Kidney/pathology*;
Liver/pathology*;
Lung/pathology*;
Male;
Myocardium/pathology*;
Proto-Oncogene Proteins c-bcl-2/metabolism*;
Random Allocation;
Rats;
Rats, Sprague-Dawley;
Spleen/pathology*;
Time Factors
- From:
Journal of Forensic Medicine
2006;22(4):241-244
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To find whether Bcl-2 and Caspase-3 take part in the pathophysiological mechanism of tetramine toxification.
METHODS:Sixty Sprague-Dawley rats were divided into normal control group, the sham poisoned group, high dose poisoned group, low dose poisoned group. High dose poisoned group were administered 1.0 mg/kg weight body tetramine by mouth, however low dose poisoned group was administered tetramine 0.1 mg/kg weight body by mouth. The rats of the sham poisoned group were administered water, and rats of normal control group were given nothing. Bcl-2 and Caspase-3 were detected by immunohistochemistry staining and the results were assessed by image analysis system.
RESULTS:The expressions of Bcl-2 and Caspase-3 in all organs were similar, ie, Bcl-2 and Caspase-3 expressed obviously in all organs of high dose poisoned group; in all organs of low dose poisoned group, they were hardly detected at 30 min after administration, however, at 3 h after administration, they could be detected obviously; Bcl-2 got to peak at 6 h-3 d after administration and Caspase-3 got to peak at 24 h-3 d after administration.
CONCLUSION:Bcl-2 and Caspase-3 take part in the pathophysiological procedure of tetramine poisoned rats.
