DNA genotyping of oral epithelial cells by laser capture microdissection.
- Author:
Li-Hua GU
1
;
Cheng ZHANG
;
Lian-Kang CHEN
;
Hui-Fen ZHEN
;
Li CHENG
;
Huai-Gu ZHOU
Author Information
1. Shanghai Institute of Forensic Science, Shanghai, 200083, China.
- Publication Type:Journal Article
- MeSH:
Cell Separation/methods*;
DNA/genetics*;
Epithelial Cells;
Genotype;
Humans;
Lasers;
Microdissection/methods*;
Mouth/cytology*;
Tandem Repeat Sequences
- From:
Journal of Forensic Medicine
2006;22(3):196-203
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:The STR genotypping of trace oral epithelial cells which are microdissected by laser capture microdissection system (LCM) is explored.
METHODS:The oral epithelial cells are microdissected using a low-power infrared laser by VERITAS Microdissection Instrument. STR loci of Profiler Plus are detected by multiplex PCR procesures.
RESULTS:DNA genotyping of 7-8 oral epithelial cells are succeeded, and DNA genotyping of 3-4 oral epithelial cells are failed.
CONCLUSION:It is viable in genotyping of trace oral epithelial cells by Laser Capture Microdissection as a new technology of seperating single cell.
