Sequences of off-ladder alleles of PowerPlex 16 kit in Chinese Han population.
- Author:
Hui-Ling LU
1
;
Yun-Chun TAI
;
Chao LIU
;
Han-Yan LI
Author Information
1. Department of Forensic Medicine, SUN Yat-sen University, Guangzhou 510089, China. luhl@mail.sysu.edu.cn
- Publication Type:Research Support, Non-U.S. Gov't
- MeSH:
Alleles;
Asian People/genetics*;
Base Sequence;
China/ethnology*;
DNA/analysis*;
Female;
Gene Frequency;
Genetics, Population;
Genotype;
Humans;
Male;
Sequence Analysis, DNA;
Tandem Repeat Sequences/genetics*
- From:
Journal of Forensic Medicine
2006;22(3):186-189
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To study the sequences of off-ladder alleles of PowerPlex 16 kit in Chinese Han population and their nomenclature.
METHODS:10071 Samples from unrelated individuals in Chinese Han population were screened by using PowerPlex 16 kit and ABI 377 or 3100. The samples showing off-ladder alleles were re-screened with PAGE and the off-ladder alleles were obtained and sequenced.
RESULTS:32 off-ladder alleles were found in 11 STR loci, whose frequencies ranged from 0.05 per thousand to 4.02 per thousand. These alleles were classified as four types: (1) having complete repeat but its length is out of the ladder; (2)having incomplete repeat; (3) having deletion or insertion of one or two base(s) in flanking sequence; (4) having deletion of some segment.
CONCLUSION:Off-ladder alleles have various types. It demonstrates varying repteating number of the core unit, and variation in the flanking sequence or core sequence as well. The nomenclature of International Society for Forensic Haemogenetics cannot define these alleles effectively.
