Observation the electroporation of blood cells in vitro by scanning electron microscope.
- Author:
Ye WANG
1
;
Min LIU
;
Xue-Mei PENG
;
Fan LI
;
Fu ZHANG
;
Kui ZHANG
;
Zhi-Gang LIAO
Author Information
1. West China Preclinical Medicine and Forensic Medicine School, Sichuan University, Chengdu 610041, China. wangyh11@163.com
- Publication Type:Journal Article
- MeSH:
Adult;
Blood Cell Count;
Cell Membrane/ultrastructure*;
Cell Membrane Permeability;
Electroporation/methods*;
Erythrocytes/ultrastructure*;
Female;
Humans;
In Vitro Techniques;
Leukocytes/ultrastructure*;
Male;
Microscopy, Electron, Scanning;
Middle Aged;
Young Adult
- From:
Journal of Forensic Medicine
2006;22(3):177-179
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To observe changes on cell membrane in blood cells after they were been electrified.
METHODS:Blood were electrified for 5, 10, 20, 30 s, 1 min respectively, and Scanning electron microscope was used to detect the changes on their cell membranes.
RESULTS:Pores were detected both on electrified erythrocytes and leukocytes with round or ellipse shapes. The erythrocytes often have one or more pores while the leukocytes often have more pores looked like cribble. The rates of perforated cells were increased with the prolonging time of electrification, 5 s with 6% and 1 min increased to 40%.
CONCLUSIONS:Alternating current can cause the cell perforating, and the rates of perforated cell were increased with the prolonging time of electrification.
